Rolling circle amplification of synthetic DNA accelerates biocatalytic determination of enzyme activity relative to conventional methods
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چکیده
منابع مشابه
Controlled Microwave Heating Accelerates Rolling Circle Amplification
Rolling circle amplification (RCA) generates single-stranded DNAs or RNA, and the diverse applications of this isothermal technique range from the sensitive detection of nucleic acids to analysis of single nucleotide polymorphisms. Microwave chemistry is widely applied to increase reaction rate as well as product yield and purity. The objectives of the present research were to apply microwave h...
متن کاملSignal amplification by rolling circle amplification on DNA microarrays.
While microarrays hold considerable promise in large-scale biology on account of their massively parallel analytical nature, there is a need for compatible signal amplification procedures to increase sensitivity without loss of multiplexing. Rolling circle amplification (RCA) is a molecular amplification method with the unique property of product localization. This report describes the applicat...
متن کاملThe Discovery of Rolling Circle Amplification and Rolling Circle Transcription.
Nucleic acid amplification is a hugely important technology for biology and medicine. While the polymerase chain reaction (PCR) has been highly useful and effective, its reliance on heating and cooling cycles places some constraints on its utility. For example, the heating step of PCR can destroy biological molecules under investigation and heat/cool cycles are not applicable in living systems....
متن کاملPractical applications of rolling circle amplification of DNA templates.
Since its recent implementation at one of the world's largest high-throughput sequencing centers, the utility of MP-RCA for DNA sequencing has been thoroughly validated. However, applications of this technology extend far beyond DNA sequencing. While many of these applications have been explored in this chapter, the future will undoubtedly add to this growing list.
متن کاملGold Nanowire Based Electrical DNA Detection Using Rolling Circle Amplification
We present an electrical sensor that uses rolling circle amplification (RCA) of DNA to stretch across the gap between two electrodes, interact with metal nanoparticle seeds to generate an electrically conductive nanowire, and produce electrical signals upon detection of specific target DNA sequences. RCA is a highly specific molecular detection mechanism based on DNA probe circularization. With...
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ژورنال
عنوان ژورنال: Scientific Reports
سال: 2020
ISSN: 2045-2322
DOI: 10.1038/s41598-020-67307-9